Subunit interactions in enzymes
نویسندگان
چکیده
منابع مشابه
Subunit interactions in bovine papillomavirus.
Papillomaviruses, members of a group of dsDNA viruses associated with epithelial growths and tumors, have compact capsids assembled from 72 pentamers of the protein L1. We have determined the structure of bovine papillomavirus by electron cryomicrosopy (cryoEM), at approximately 3.6 A resolution. The density map, obtained from single-particle analysis of approximately 4,000 particle images, sho...
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The 19,000-dalton light chain (LC2) can be completely and reversibly removed from chicken pectoralis myosin in 1 mM EDTA and 5 mM ATP using immunoaffinity chromatography at 37 “C. Earlier methods have led to only partial removal of LC2 or have caused limited degradation of the heavy chain. Electron microscopy of LC2-deficient myosin showed it to have a marked tendency to aggregate into oligom...
متن کاملThe subunit interactions of fumarase.
The subunit interactions of fumarase have been examined as a function of enzyme concentration, pH, and concentration of guanidine hydrochloride. Fumarase was found to dissociate, with loss of activity, either at low concentrations of the enzyme ( 1 M). At pH 9 a species of enzyme was produced which was ...
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The pH-dependent dissociation of porcine heart mitochondrial malate dehydrogenase (t-malate:NAD+ oxidoreductase, EC 1.1.1.37) has been more extensively characterized. "he native, dimeric form of the enzyme (Mr = 70,000) which exists at pH 7.5 has previously been shown to dissociate into its constituent subunits (Mr = 35,000) at pH 5.0 (Bleile, D. M., Schulz, R. A., Gregory, E. M., and Harrison,...
متن کاملUnderstanding allosteric and cooperative interactions in enzymes.
The paper that introduced biochemists to the idea of allosteric feedback inhibition [Monod J, Changeux J-P & Jacob F (1963) J Mol Biol 6, 306-329] is now 50 years old, and the two papers on models for enzyme cooperativity that followed it [Monod J, Wyman J & Changeux J-P (1965) J Mol Biol 12, 88-118; Koshland DE, Némethy G & Filmer D (1966) Biochemistry 5, 365-385] are almost as old. All of the...
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ژورنال
عنوان ژورنال: Nature
سال: 1976
ISSN: 0028-0836,1476-4687
DOI: 10.1038/260082b0